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回答
蛋白纯化文献

文献里写的equilibrated in 10 mM Mes (pH 6.0). Adsorbed proteins were eluted with a 500-ml salt gradient from 0 to 0.5 M NaCl/10 mM Mes, pH 6.0, at a flow rate of 2 ml/min.是什么意思?怎么理解洗脱?