INTRODUCTION

Kidney transplantation is considered the gold standard of treatment for most patients with end-stage renal disease, nevertheless kidney transplant recipients (KTRs)are characterized by long-term clinical complications and high risk of cardio-vascular disease (CVD).

通过认知微网技术领域的研究热点,有助于更好地把握整个行业的发展动向,使相应行业人员明确领域发展态势,为下一阶段微网领域的研究提供更具有意义的参考性建议。运用CiteSpace软件对检索到的期刊文献展开关键词共现分析,生成微网可视化技术图谱,并定位微网技术热点。

In addition to the traditional CVD risk factors (e.g.,hypertension, dyslipidaemia, diabetes mellitus) other non-traditional factors influence the high incidence of cardiovascular events (e.g., duration of prior dialysis, graft function after transplantation, elevated inflammatory markers, proteinuria, toxic effects of immunosuppressant drugs, bone mineral metabolism abnormalities and vascular calcifications). However, among all these risk factors, the lack of physical exercise and a sedentary lifestyle seem to play crucial roles[1].

实验室是高校进行实验教学的重要基地，为高校的实验教学提供了重要场所。学生通过实验教学能够提高自己的实验能力，更深入地将理论和实验相结合，更容易培养学生的创新能力，提高学生的综合素质。实验教学仪器是实验室必不可少的物品，也是实验教学的基础设备，所以，加强实验教学仪器设备的管理对提高教学质量有着重要意义。

There is mounting evidence that physical exercise reduces the risk of all-cause mortality[2,3] and it is effective in the primary and secondary prevention of CVD in the general population[4]. Physical activity is also considered a key element in the prevention and management of chronic diseases[5], including Chronic Kidney Disease (CKD).

After transplantation, patients are expected to be more active than before because their uremic syndrome is corrected and they do not have to do haemodialysis treatment[6]. However, their cardiorespiratory fitness remains reduced by 30% in comparison with matched control subjects[7]. Only in selected cases they can achieve results comparable to a healthy population[8],but not all patients increase their physical activity after transplantation; thus, the majority of KTRs maintain a sedentary lifestyle, often associated with an increase in body fat and weight gain[9].

Whether exercise can positively affect outcomes in KTRs has only been addressed in few studies[4],with a small number of subjects and with different types, intensity and durations of interventions lasting almost always not more than six months[10,11]. In some studies, exercise was carried out tightly at home without direct supervision and with a partial adherence to the intervention[6]. Furthermore, few studies have investigated the effect of a combined aerobic and resistance training[4], and the effect of these protocols on kidney function is rather unknown.

In this paper, we present some clinical and fi tness outcomes of a 12-mo study conducted on KTRs, with the aim to evaluate the potential effects of supervised exercise combining aerobic and resistance training.

既有专家学者，也有实际工作者在呼吁，农村富裕之后，当下一件急迫的事情，是按照国家乡村振兴战略和社会主义核心价值观厚植乡村人文精神，重塑乡村伦理道德体系，大兴文明乡风，如习近平总书记所言：“乡村振兴，既要塑形，更要铸魂。”

MATERIALS AND METHODS

Organisational model

We introduced a project, based on a model of cooperation among: (1) Transplantation specialists (surgeons and nephrologists), who selected patients suitable for physical activity; (2) sports physicians who prescribed a personalised exercise programme based on the results of functional assessment tests; (3) exercise specialists who supervised the patients performing the prescribed programme. This organisation aims to check the patients from clinical and functional perspectives and to identify facilities in their home districts where patients can easily perform their training programmes under supervision[12,13].

Study design

This is a multicentre, controlled, prospective, nonrandomised study that considered the enrolment of KTRs patients with clinical and functional stabilities.

Inclusion criteria were the 18-60 years age range,and at least six months after organ transplantation;exclusion criteria were orthopaedic limitations,psychiatric or neurological disorders, proteinuria within nephrotic range, poor compliance to treatment and any cardiovascular contraindication to exercise testing and training.

Patients were divided into an interventional exercise group (Group A), in which personalised training was supervised, and a usual care group (Group B), in which some exercise indications were given without a specific prescription and supervision. All subjects received individualised counselling by the transplant centre regarding the protocol, and the inclusion in Group B was based on logistic and organisational grounds(patients living in regions not taking part in the project or living in areas without sports medical centres or an accessible gyms). This is the practical reason why we adopted a non-randomised design of our study.

Blood chemistry and urinalysis, complete blood count, and a cardiac evaluation were performed by the transplantation centres to assess the exclusion criteria.After the administration of the SF-36 questionnaire to evaluate Health-Related Quality of Life (HRQoL), the patients were sent to the sports medicine centre to perform the functional assessment tests for exercise capacity, muscle strength, and body composition.

旋风分离器是通过气固两相流体从而实现旋转运动，并在离心力的作用下将固体颗粒从气流中进行有效分离的设备。可能会由于工作人员的安装和调试以及运行经验不足等问题，导致旋风分离器在运行的过程中出现堵塞的情况，如果没有及时发现并进行疏通操作，就会出现床温急剧升高，气温快速下降的情况，这样的情况非常容易导致床层或分离器结焦等问题，导致机组被迫停运。通过对75t/hCFB锅炉运用情况的分析和调试，总结出了有效解决和增加旋风分离器效率的办法。

Based on the results of these tests, the sports physicians prescribed a tailored training programme only for Group A. Then, patients in Group A were sent to a certified gym to start the prescribed training under the supervision of exercise specialists, while patients in Group B, as usual, were provided general information to encourage regular physical activities at home but no speci fi c prescription and supervision were given.

Both groups were checked at baseline (T0), six(T6) and 12 mo (T12) from the enrolment. The trial did not envisage any change in the immunosuppressive treatment (Table 1).

Written informed consent was obtained by the patients before inclusion, according to the procedures approved by the Ethics Committee. This trial was registered in the ISRCTN registry (Trial ID: ISRCTN66295470) and was conducted in compliance with the ICH Guidelines for Good Clinical Practice, the Helsinki Declaration and national rules regarding clinical trial management.

Supervised training intervention (Group A)

The exercise prescription included sessions of aerobic and resistance training. The total duration of each session was one hour, with a frequency of 3 times per week for 12 mo. In every session, the aerobic training was performed on a stationary bike and was administered with an intensity corresponding to the lactate aerobic threshold[14], previously assessed by the incremental cycling test at T0 for the first six months and at T6, for the subsequent period. The intensity was continuously monitored by heart rate monitors (Polar,Finland) allowing the patients to maintain a constant heart rate (HR) corresponding to the aerobic threshold during the aerobic training.

In the same session, the subsequent resistance training consisted of two sets of 20 repetitions at 35%of one Repetition Maximum (1RM) for each musclegroup of the upper (elbow flexors, elbow extensors,shoulder abductors) and lower limbs (knee extensors,plantar flexors). The training intensity at 35% of 1RM was chosen to increase local muscle endurance considering that KTRs are novice individuals for strength training where learning proper form and technique is paramount[15,16]. Resistance training was not performed with the upper limb with arterio-venous fistula. Warmup, cool-down and stretching exercises were included in all training sessions. The intensities of aerobic and strength trainings were adjusted after the T6 assessment.

Non-supervised home-based exercise intervention(Group B)

Group A showed a significant average improvement in maximum workload and V’O2 peak at T6 (P = 0.0010,P = 0.0370), and the levels continued to increase at T12 (P = 0.0003, P = 0.0099) compared to Group B(Table 3).

The International Physical Activity Questionnaire(IPAQ) short-version[17] was administered only to Group B at the three-time points to evaluate the level of physical activity through nine items that provide information on the time spent walking, in vigorousand moderate-intensity activity and in sedentary activity. This questionnaire assessed the actual level of daily physical activity and thus reduce the bias between the two groups.

Primary outcomes

Renal function, lipid values and blood chemistry:In both groups, creatinine (mg/dL) using the Jaffè method, estimated glomerular filtration rate (eGFR)using the chronic kidney disease epidemiology collaboration (CKD-EPI) equation, proteinuria (mg/1000 mL)using the turbidimetry method reported in g/24 h calculating 24-h urine collection were collected to check the renal function at T0, T6 and T12.

党的十九大报告指出我国经济正由高速发展阶段转向高质量发展阶段，如何引导金融资本服务实体经济成为当前金融改革的重要任务。以生物科技、智能制造、信息技术为代表的战略新兴产业成为高质量发展的核心动力，其发展状况成为影响经济转型成功与否的关键因素。相对于传统行业，战略新兴产业投资周期较长、研发风险较大并且技术门槛较高。由此，企业与外部投资者之间存在较大的信息不对称问题，在以信贷为主导的传统融资模式下面临较为严重的融资约束。

Total cholesterol and triglycerides were measured from venous blood sample using flow cytometry and light-scattering methods to evaluate lipid metabolism.Haemoglobin and glycaemia values were also measured.

Exercise capacity: Exercise capacity was assessed by an incremental cycling exercise starting from a 5-min unloaded cycle and increasing by 20 W every four minutes until the subject was unable to continue. A 12-lead electrocardiogram was monitored continuously throughout the test. At each step a capillary blood sample from the earlobe was taken to measure blood lactate concentration (YSI 1500-Sport; Yellow Springs,United States) to estimate the workload corresponding to aerobic and anaerobic thresholds, conventionally declared at 2 and 4 mmol/L of lactate, respectively[14].Systolic and diastolic blood pressures were measured with sphygmomanometers at rest, at each step and at the third minute of recovery.

Oxygen uptake (V’O2) was determined continuously using an open-circuit spirometry system (Sensor Medics, Anaheim, United States), and the V’O2 at the highest tolerated workload was determined and was referred to as V’O2 peak (mL O2/kg per minute).

Muscular strength and power: A leg press (Technogym,Cesena, Italy) and free weights were utilised to assess the dynamic muscular strength of the lower and upper limbs (knee extensors, plantar flexors, elbow flexors, elbow extensors and shoulder abductors). The 1RM strength was calculated using an indirect method consisting of 7 to 12 repetitions with submaximal loads[18].

The general strength was measured using a handgrip dynamometer (Lafayette, IN, United States).

The power of the lower limbs was measured indirectly from the fl y time of a countermovement jump(CMJ) and was expressed as maximum displacement(m) of the centre of mass during fly (Optojump,Microgate, Italy).

Secondary outcomes

BMI and body composition: Body mass index (BMI)was calculated using the ratio between weight and square height (kg/m2).

The KTRs included in Group B who received only general information to promote regular physical activity at home, without a specific supervision, did not show any improvement in physical fitness outcomes,indicating a low adherence to non-supervised homebased physical activity. This demonstrated that without a direct or indirect supervision (e.g., follow-up by calls or e-mails), patients tend to not carry out physical activity even if it is recommended by the physician.

职业素养的传授形式大多数采用是的PPT讲解模式，和传统的教学模式相当，造成学生学习枯燥无味，觉得和自己的关联性并不是很大，现代的高校都在各种教学改革，提高教学效率，由此职业素养渗透到专业课中，也必须要改变讲授的模式，让学生更深刻的掌握职业素养在大学生今后的道路当中的重要性。

Health-related quality of life: The 36-Item Short Form Health Survey (SF-36, Medical Outcomes Trust)was used to evaluate self-reported domains of health status[20] completed by the patients independently at T0, T6 and T12.

Statistical analysis

The sample size to assess eventual differences in exercise capacity, muscular strength, renal function,BMI and HRQoL was determined using the Software G-Power (version 3.1.9.2) with an alpha level of 0.01 and a power of 0.90. All descriptive data are presented as the mean ± standard deviation (SD). Linear mixedmodels were used to assess the effects of time and group on dependent variables, with T0 and Group B set as the base categories. Random intercepts were used for individual subjects. Significance was set at P ＜0.05, and the raw coefficients for the fixed effects and interactions are reported with 95%CI. The statistical analysis was performed using R software for Windows(v. 3.2.3).

利用改进的Jensen尾流模型计算位于下游的风机所捕获的风速。简化的尾流效应模型如图1所示。在大多数研究中,图1所示的锥形区域为风通过每个单独的风机时所形成的尾流效应区域。本文将其简化为二维模型[14]。

RESULTS

Subjects

Ninety-nine KTRs were recruited by nine transplant centres between January 2011 and June 2015. Fiftytwo patients were included in Group A, and 47 in Group B. Eight patients from Group A decided to withdraw and were considered dropouts. The causes were economic problems and lack of motivation (n = 4)or work conflicts (n = 4). In Group B, six patients did not show up to the functional assessments during the follow-up.

Forty-four KTRs from Group A (21 female and 23 males, mean ± SD age 47 ± 12 years, mass 69 ± 14 kg,BMI 24.1 ± 4.3 kg/m2, time from transplant 5.5 ±7.1 years, dialysis vintage 36 ± 35 mo, range 1-156)and 41 KTRs from Group B (13 female and 28 males,age 49 ± 9 years, weight 75 ± 13 kg, BMI 25.5 ± 4.4 kg/m2, time from transplant 3.6 ± 4.0 years, dialysis vintage 33 ± 34 mo, range 1-144) were analysed.There were no significant differences between groups regarding: age (P = 0.35), BMI (P = 0.16), time from transplant (P = 0.11), and dialysis vintage (P = 0.42).The only significant difference was found for body mass(P = 0.02).

Pathologies leading to renal disease and immunosuppressive therapies of the patients are shown in Tables 1 and 2 respectively.

Exercise program adherence

In Group A, the exercise program adherence, defined as a total number of exercise sessions completed as proportion of total possible number of session (144 sessions) during the 12-mo period was 93% ± 6%.None adverse events were reported.

Primary outcomes

Creatinine tended to decrease in Group A at T12 and increase in Group B at the same time, but the results were not significant. No significant changes were found in eGFR or proteinuria in either group.Average triglyceride and cholesterol levels showed slight changes at T12 in both groups which were not significant (Table 3).

Only three patients (one in group A and two in group B) were diabetic under insulin therapy (Table 2). In both groups, glucose values were always ＜ 126 mg/dL without significant changes between the three time points.

Diastolic and systolic blood pressures were similar(P ＞ 0.05) in the two groups at rest, at the maximum workload and after three minutes of recovery, at T0, T6 and T12 (Table 3). The only significant difference was found between groups for systolic blood pressure at the third minute of recovery, that was always lower in Group A (P = 0.0489).

At T0 and T6 patients in Group B were provided general information to encourage regular physical activities at home, as usual by the transplant centre, but no specific prescription and supervision were given.

The maximum HR, anaerobic threshold workload and corresponding HR significantly increased at T12 (P＜ 0.05) in Group A compared to Group B. In Group B, we found a significant decrease in the anaerobic threshold HR from T0 to T12 (P = 0.0434). No additional significant differences were found in Group B at T6 and T12 in any variables (Table 3).

Another limit is due to the workloads chosen for the aerobic and strength trainings. We adopted a steady state aerobic exercise protocol at intensity corresponding to the lactate aerobic threshold. Different training protocols, i.e., interval training, or different duration of the sessions could be more effective.Furthermore, it is possible that with a higher percentage of 1RM and with a different progressive strengthening protocols the improvements would be higher, especially when training the upper limbs. The fact that the upper limb with the arteriovenous fistula was not trained,for safety precautions, also affected the final strength results. Furthermore, we checked both groups after 6 mo of training, so probably a more frequent adjustment in the prescription of the relative intensity of trainings would lead to better functional outcomes in Group A or would give further motivations in Group B.

Group A showed a significant improvement in the handgrip test at T12 (P ＜ 0.05) compared to Group B(Table 3).

马克思主义唯物史观强调生产力是人类利用和改造自然的能力，是人类生存依托的物质基础。生产力由劳动资料、劳动对象、劳动者和科学技术等要素构成。生态环境所涉及的各个方面与生产力都息息相关。随着走向生态文明新时代，生态环境越来越成为重要的生产力。习近平总书记强调：“要正确处理好经济发展同生态环境保护的关系，牢固树立保护生态环境就是保护生产力、改善生态环境就是发展生产力的理念。”[5]

Group B showed a significant increase in elbow flexor, elbow extensor and shoulder abductor strength(respectively, P ＜ 0.05) at T6, but the levels remained below the values of Group A (Table 3).

No changes were found in Group B in the level of daily physical activity assessed by IPAQ, which remained at a low level (＜ 600 MET per minute per week) at the 12 mo follow-up. Theoretical IPAQ calculated from the exercise protocol performed by Group A was ＜600 METper minute per week at baseline, and ＞ 600 METper minute (range 1215-1413 MET per minute) per week at T6 and T12 (P ＜ 0.01).

Secondary outcomes

Group A showed a significant decrease in BMI at T12 (P= 0.0013) and fat mass percentage at T6 (P = 0.05)compared to Group B (Table 3).

In HRQoL, significant improvements were found in Group A in physical function scale at T6 (P = 0.0082)and continued to increase at T12 (P = 0.0019), in rolephysical and social functioning scales at T12 (P = 0.0321,P = 0.0346) compared to Group B, in which we found no significant changes in any scales (Table 4).

(1)对患者术前术后行Eckardt评分，包括体重、吞咽困难、胸骨后疼痛、反流，评分范围为0～12分(表1)。术后Eckardt症状评分≤3分，则认为手术有效；(2)食道测压检测：检测术前术后的LES静息压、松弛压，比较较术前是否明显下降;(3)对患者术前术后行SF-36[6]健康问卷调查，比较术前术后生活质量是否提高。综合评分的参考值范围：良好116.8～141.4，中等72.1～116.6，差65.2～71.0。

DISCUSSION

In relation to anthropometric measures, the 12-mo supervised programme combining aerobic and resistance training was effective in reducing BMI[33] and fat mass in Group A, with a non-significant reduction in fat-free mass (-2%). However, in our study, the lipid pro file remained the same; 39% of patients were taking a statin or ezetimibe as a regular drug (Table 2), which would make further improvement in the lipid pro file unlikely. Moreover, the patients did not receive a diet programme.

Fat mass (FM) percentage was determined using the Jackson and Pollock body density equation considering seven skinfolds in both men and women (abdominal,thigh, triceps, bicep, subscapular, suprailiac, chest)measured with a Harpenden calliper[19] at T0, T6 and T12.

van Adrichem et al[21] highlighted how perceived barriers of physical activity in KTRs such as physical limitations, lack of energy, and comorbidities cannot be omitted. Moreover, the lack of specific counselling by physicians about the benefits of physical activity is a critical issue. However, in the present study we recorded a dropout rate of 15% in Group A and 13%in Group B. Painter et al[6] reported a dropout rate of 33% at one year in their exercising group of patients who performed home-based training with regular phone follow-up. Greenwood et al[22] in their 12-wk study reported a dropout of 7 on 20 KTRs (35%) in both aerobic and resistance training supervised groups.Most of these patients reported difficulties attending classes following return to work after transplantation.Riess et al[4] reported a dropout of 2 out of 16 (13%)on their 12-wk study in the supervised exercise group and 1 out of 15 (7%) in the home based usual caregroup. O’Connor et al[23] in an un-supervised period of self-managed physical activity reported an attrition rate of 30% at the 12 mo time point that con fi rms a low exercise adherence without supervision.

Compliance with the treatment is a common barrier of health programmes based on exercise even if transplant recipients who have experienced a supervised exercise programme supported that it was beneficial to health and well-being[24]. Social, cognitive,personality, environmental, and socio-economic factors,unrelated to the recommended guidelines, seem to be of greater importance in considering behavioural adherence issues[25] in KTRs. To improve physical exercise programme compliance and longer-term outcomes, strategies to diversify and stimulate exercise training or change elements of training like introduce specific tracking devices designed for KTRs should be examined. Anyway, data from our study clearly show that recommendations alone are not sufficient to induce a change in lifestyle and physical fitness. On the other hand, the supervised training for long periods is costly and cannot be proposed for all the transplanted patients,so it is urgent to study new solutions, starting from the cooperation between transplantation, sports medicine,and exercise specialists.

Renal function data, expressed as creatinine, eGFR and proteinuria, were compatible with the framework of patients of a select population undergoing successful renal transplantation. The proposed training protocol had no negative effects on the renal function in the medium term and, more in detail, creatinine values tended to decrease in Group A and to increase in Group B at the same time, but the results were not significant after 12 mo. The tendency to decrease of creatinine and eGFR in Group A can be considered as positive effect of physical exercise and needs specific studies. Patients in Group A did not show any significant increase in muscle mass after 12 mo of resistance training such as to affect creatinine and eGFR, probably because of the low intensity of the resistance training.

To the best of our knowledge, 12 mo of observation period is one of the longest in the literature with reference to aerobic and resistance trainings; however,it is a relatively short-time period and further studies with larger populations are necessary to understand the long-term effects of exercise or sedentary lifestyle on the renal function of KTRs.

Regarding exercise capacity, in Group A we observed a 12% increase of V’O2 peak at T6. Similar results were obtained by van den Ham et al[26] in 33 KTRs after 12 wk of combination of endurance and strength training in which V’O2 peak increase of 10%(from 21.6 ± 6.3 to 23.8 ± 6.1 mL/kg per minute).Riess et al[4] reported an increase of V’O2 peak (from 20 ± 9 to 23 ± 10 mL/kg per minute) after 12 wk of supervised endurance training (three times/week) on cycle ergometer at 60%-80% of V’O2 max involving 16 patients.

In a study of eight KTRs, Romano et al[27] utilised a supervised interval training technique for 10 wk, 40-min sessions for three times per week. They reported an increase of 13% of V’O2 peak.

Another intervention was published by Kempeneers et al[28] who trained 16 KTRs for six months in preparation for the National Transplant Games. Their mean V’O2 peak rose from 29.0 ± 7.8 to 37.5 ± 4.8 mL/kg per minute, with an increase of 27%.

In the other hand, Painter et al[6] prescribed an individualised home-based exercise training programme in 54 KTRs, consisting on 30 min, four times per week of training at an intensity corresponding to 60%-80%of maximal HR. Patients were contacted every two weeks by phone to assess progress and adherence to the programme and to adjust it as needed. After six months, V’O2 peak increased from 24.0 ± 7.5 to 27.8± 11.0 mL/kg per minute (+ 16%) and to 30.1 ± 10.3 mL/kg per minute (+ 25%) after 12 mo.

We can conclude that the aerobic training in KTRs leads to a substantial increase in aerobic power[29]. In most cases, the type of training meets the minimal clinically significant difference of 3.5 mL/kg per minute(i.e., 1 metabolic equivalent), which is associated with improved outcomes in CVD. However, Riess et al[4] after 12 wk of endurance training were unable to demonstrate any change in resting small or large arterial compliance, peak exercise systemic vascular resistance and Framingham Risk Assessment Score,indicating that exercise intensity and overall duration are probably the most critical factors affecting CVD risk pro fi le.

Our study did not reveal significant differences in blood pressure between the two groups, both showing normal blood pressure at rest, during and after maximal incremental exercise. This may be explained by the tightly controlled anti-hypertensive regimes in post-transplant care, as previously reported in other studies[23].

Reduced general muscular strength has been related to an increased risk of all-cause of cardiovascular mortality[30] and the handgrip test values are a recognised marker of health status[31]. In our study,the handgrip test values improved after six months of training and significantly improved after 12 mo,whereas in the Group B we found a trend in reduction in strength, even if it was not significant. Moreover, in the Group A the muscular strength of the lower limbs improved, and the power of the lower limbs increased after 12 mo. This increase in maximal strength and CMJ values may be associated with both neural adaptations and muscle trophism improvement[32]. This finding is consistent with prior studies[6].

教学形式单一化是一个不仅仅存在于小学语文阅读教学当中的教学问题，在其他阶段和其他科目的教学中也有出现过。教师在进行小学语文阅读教学时总是喜欢按照模板教学，先让学生阅读课文，再自己讲解课文。这种教学模式虽然能节省课堂时间，但是却并没有在真正意义上提高课堂阅读效率，学生在这样的课堂里只能被动接受，无法通过自己思考理解来完成学习。

The main result of this study is that in selected KTRs, a programme of 12 mo of supervised training performed one hour, three times per week in certified gyms does not affect the renal function, leading to significant improvement in aerobic fitness, muscle strength and HRQoL, with a significant decrease of BMI. Furthermore, the proposed organizational model led to a high exercise program adherence, i.e., to a positive change in lifestyle.

Regarding quality of life, we found significant improvement in Group A in the self-perception of physical function, role-limitation to physical activity and social function. The KTRs in the usual care group (Group B) did not show any improvement in HRQoL scores.This finding confirms that supervised exercise training led to a better self-perception of quality of life[34].

The association of aerobic and resistance training was safe; no acute cardiovascular event, renal graftrelated or serious adverse events due to endurance or strengthening exercises were recorded. The inclusion in the protocol of the CMJ test did not have any consequence to the musculoskeletal system, indicating that KTRs can safely perform supervised power exercises[35]. The accurate selection of the patients and the cardiovascular assessment at T0 certainly contributed to these findings.

The present study has some limitations. First, it is a non-randomised study; we included the patients in the usual care group (Group B) on logistic and organisational grounds. Therefore, the two groups were different in baseline assessments of body mass.

Group A showed a significant average improvement in lower limb strength and power expressed by an increase in plantar flexor muscle strength (P = 0.0368)and CMJ (P = 0.0293) at T12 compared to Group B. No significant differences were found in Group B at T12 in any variable (Table 3).

The anthropometric assessment by the skinfolds technique has some limitations and probably it would be possible to detect significant changes with more precise methods (i.e., dual energy X-ray absorptiometry).Another limit is that we administered the IPAQ only to Group B to reduce the bias between the two groups,but it was impossible to make any direct comparison with Group A.

(3)表述不同,即存在指代的两个要素在文字表达上不同,两个要素间可能一个是另一个的别名,或者根据上下文,两个要素都指向同一个实体,例如“中华人民共和国”←“中国”,“修理巷道的20名矿工”←“被困人员”;

Finally, patients included in our study were carefully selected and were thus not representative of the entire KTRs population. Furthermore, the prescribed exercise program was based on blood tests and other measurements that are not routinely performed in gyms and cannot be universally applied, so the generalizability of the results is limited.

在农业基础设施建设过程中，小型农田水利工程的建设是提升农业综合生产能力的关键前提条件，也是我国小康社会建设的关键保障基础。在这一前提下，小型农田水利工程的建设，为我国农业发展以及农村经济水平的提升起到了很好的助推作用，继而提升了粮食生产能力[1]。另一方面，由于我国经济水平的限制，传统的农田水利建设工作开展质量并不理想，大部分农田水利基础设施已经退化，亟待进行改进和更新，这也是小型农田水利工程建设的重要性所在。

Selected KTRs can safely perform training protocols lasting 12 mo, with association of aerobic and resistance exercises.

Despite some limitations, this paper shows that developing a supervised exercise protocol for KTRs is a useful and safe non-pharmacologic contribution to usual after-transplant treatments, which can improve the physiological variables related to physical fitness and cardiovascular risks without consequences on renal function. Our study is an example of collaborative working between transplant centres, sports medicine and exercise facilities, aimed to apply the concepts of“exercise is medicine”.

Further studies with longer follow-up and larger populations are necessary to understand the strategies that will improve adherence to training programmes,control costs and lead to steady and durable lifestyle changes in KTRs.

AARRTTICICLLEE HHIGIGHHLLIGIGHHTTSS

Research background

Kidney transplant recipients (KTRs) are characterised by long term clinical complications and high risk of cardiovascular disease. After transplantation,physical activity is considered a key element in the prevention and management of chronic diseases, however the majority of KTRs maintain a sedentary lifestyle, often associated with an increase of body fat and weight gain. Whether exercise can positively affect outcomes in KTRs has only been addressed in few studies, with a small number of subjects and with different types, intensity and durations of interventions lasting almost always not more than six mo.Furthermore, few studies have investigated the effect of a combined aerobic and resistance training, and the effect of these protocols on kidney function is rather unknown. In this paper, we present some clinical and fi tness outcomes of a 12-mo study conducted on KTRs, with the aim to evaluate the potential effects of supervised exercise combining aerobic and resistance training.

Research frontiers

Developing a supervised exercise protocol for KTRs is a useful and safe non-pharmacologic contribution to usual after-transplant treatments, which can improve the physiological variables related to physical fitness and cardiovascular risks without consequences on renal function. Further studies with longer follow-up and larger populations are necessary to understand the strategies that will improve adherence to training programmes, control costs and lead to steady and durable lifestyle changes in KTRs.

Innovations and breakthroughs

●治理土壤重金属污染，是一道世界难题。有机硅治理土壤污染重金属功能肥，5年来在南方十几个地区2万亩重金属污染土壤的试验示范，降镉率达到80%以上。经农业农村部组织专家评价，有机硅功能肥治理土壤重金属污染达到国内领先、国际先进水平。

Applications

The collaboration between transplant centres, sports medicine centres and exercise facilities is effective to prevent the low adherence to suggested and/or prescribed physical activity.

Terminology

Oxygen uptake (V’O2) at the highest tolerated workload was referred to as V’O2 peak (mLO2/kg per minute).

ACKNOWLEDGMENTS

The authors gratefully acknowledge Paola Di Ciaccio,Claudia Carella, Claudia Ferraro and Carlo Donati for their cooperation in the project.

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